Contact: Department of Biochemistry, University of Benin, Benin City
The anti-diarrheal effect of aqueous extracts (dried and fresh forms) of Morinda lucida leaves was evaluated in Wistar rats. Albino Wistar rats (25) with average weight (120 ± 5) g labelled A-E were divided equally into five groups. Each rat in the groups apart from group A was orally administered 12 x 108 CFU/ml saline diluted Sd1 cells. Sd1-induced diarrhea was confirmed by the presence of bloody stool with mucus and fetid odour. Administration of the extracts started 48hrs after induction. Groups A were administered normal saline, B; 500mg/bw of fresh extract of M. Lucida, C; 500mg/bw of dried extract of M. Lucida, D; 7mg/kg bwt of ampicillin and E was untreated. The treatment lasted for seven days. The animals were then sacrificed and blood collected in EDTA bottles for analysis. Our findings shows that Sd1- induced diarrheic rats significantly decreased the activities of Na+/K+- ATPase, Mg2+- ATPase and Ca2+- ATPase activities. There was however no significant difference in Na+/K+- ATPase, Mg2+- ATPase and Ca2+- ATPase in the groups administered dried extract and ampicillin. Significant increase in the plasma levels of these glucose and protein but a decrease in total lipid were also observed in diarrheic rats. The administration of the extracts and ampicillin significantly restored parity when compared with the control. M. lucida leaves extract demonstrated strong effect as anti-diarrheal agent.
Keywords: Anti-diarrheal, Shigella dysenteriae type 1, ATPases